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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
广州市左克生物
- CAS号:
//
- 规格:
100T
MCE NAD+/NADH 检测试剂盒(WST-8 法)是无需从样品中纯化 NAD+/NADH,基于 WST-8 的显色反应,通过比色法检测细胞、组织或其他样品中氧化型辅酶 Ⅰ (NAD+) 和还原型辅酶 Ⅰ (NADH) 各自的含量、比值及总含量的试剂盒。
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time: 2 x 1 ml CHIP lysis buffer 2 x 1 ml CHIP
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
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