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- 技术资料
- 库存:
50
- 供应商:
广州市左克生物
- CAS号:
//
- 规格:
1mL/10mL
| 规格: | 1mL | 产品价格: | ¥209.3 |
|---|---|---|---|
| 规格: | 10mL | 产品价格: | ¥1480.7 |
MCE 蛋白酶磷酸酶抑制剂,通用型是一种质谱兼容的通用型蛋白酶和磷酸酶抑制剂混合物。本产品可以有效抑制常规的细胞或组织提取物中的各种蛋白酶和磷酸酶。
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of ice-cold PBS containing protease inhibitor cocktail. 5. Prepare 0.2X trypsin diluted in PBS, and add 5 mL of it to the cells. Incubate in 5% CO2 for 5 min at 37°C. 6. Neutralize the trypsin with 10 mL of 10% fetal bovine
RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells
(Invitrogen, Catalog #25530-049) Complete tablets (protease inhibitor cocktail), EDTA-free (Roche, Catalog #04693132001) Protein A/G-PLUS Agarose (Santa Cruz Biotechnology, Catalog #sc-2003) Superscript III reverse transcriptase (Invitrogen
in microcentrifuge tubes and stored at –20°C until ready for use. Thaw on ice prior to extracting cells. The Cell Extraction Buffer must be supplemented with 1 mM PMSF (not included) and Protease Inhibitor Cocktail (not included) just prior to use to make
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