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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
广州市左克生物
- CAS号:
9014-25-9
- 规格:
50mg/100mg
| 规格: | 50mg | 产品价格: | ¥1275.0 |
|---|---|---|---|
| 规格: | 100mg | 产品价格: | ¥2040.0 |
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文献和实验Acid Phenol Yeast RNA Preparation
9. Spin 5 min at 4 deg and transfer top layer to a new tube.10. Repeat acid phenol extraction until the interface is clean (~4 times).11. Extract once with ~400 microliters CHCl3. Transfer top layer to a new eppendorf tube.12. Ethanol precipitate by adding
cold water and transfer to 15 ml conical tube. Centrifuge 4 min @ 4 deg. Remove supernatant. Quickly resuspend pellet in 800 microliters cold TES and freeze in dry ice or liquid N2. 3. Warm acid phenol in H2O bath to 65 deg. 4. Thaw cells briefly
Replication timing by density transfer
-Aldrich (previously from Isotec, which is now part of Sigma-Aldrich) "-N" medium =1.61 g yeast nitrogen base w/o amino acids and ammonium sulfate, 11.1 g succinic acid, 6.67 g NaOH per litre. Pronase : We use
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