- ￥198.05 - 2125
- MCE
- HY-116794
- 2026年01月23日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
广州市左克生物
- CAS号：
34634-22-5
- 规格：
1mg/5mg/10mg/25mg/50mg/100mg
| 规格： | 1mg | 产品价格： | ￥198.05 |
|---|---|---|---|
| 规格： | 5mg | 产品价格： | ￥425.0 |
| 规格： | 10mg | 产品价格： | ￥595.0 |
| 规格： | 25mg | 产品价格： | ￥1020.0 |
| 规格： | 50mg | 产品价格： | ￥1487.5 |
| 规格： | 100mg | 产品价格： | ￥2125.0 |
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文献和实验Native purification of His-tagged Proteins from Sf9 cells
Protocol is for 150 ml of cell culture. 1. Spin down cells and wash 1X in 10 ml ice cold PBS. 2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.0 1 % NP-40 1 mM PMSF 1 µg/ml leupeptin 3. Transfer to oakridge tubes and sonicate
GPCR Expression Using Baculovirus-Infected Sf9 Cells
promoter-driven expression of a C-terminal 6�histidine-tagged mammalian M2 muscarinic receptor in Sf 9 cells. Results demonstrate that expressed receptor could be detected and quantified using radiolabeled ligand binding, that expression was maximal
Purification of Recombinant p53 from Sf9 Insect Cells
We describe a method for purifying recombinant p53 from baculovirus infected cells in one step by anion exchange chromatography. The p53 is full-length with no flanking sequences and its expression is driven by the baculovirus polyhedron
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