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- 2026年01月23日
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- 库存：
50
- 供应商：
广州市左克生物
- CAS号：
//
- 规格：
1mg
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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
and stoichiometry for tubulin labeled with tetramethylrhodamine (TMR) NHS ester: Tubulin concentration = [(A280 - Contribution of dye to A280 ) x Dilution Factor] / Extinction coefficient of tubulin at 280 nm TMR concentration = (A555 x Dilution Factor
Coupling Peptides To Resin For Affinity Purification
5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate
Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
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