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- HY-Y0344D
- 2026年01月23日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
广州市左克生物
- CAS号：
7647-14-5
- 规格：
100g
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文献和实验CALCIUM CHLORIDE TRANSFORMATION OF BACTERIA WITH PLASMIDS
Preparation of Transformation Competent DNA Dilute an overnight bacterial culture 1:20 in L broth. (For E. coli strain JA221, at A ) Using a serological pipet, resuspend the bacterial pellet gently in 1/2 the culture volume of cold 50 mM CaCl
Cesium Chloride Purification of T7
with a drop from a master phage stock. Continue to shake cells in the water bath at 30°C until culture clarifies. Be careful not to let culture sit for long (>15 minutes) after culture clarifies . Add NaCl to the lysate to make the final concentration
Preparing Overnight Bacterial Culture
Materials: Sterile LB medium (Luria-Bertani Medium) with or without antibiotic: water 500 ml bacto-tryptone 10 g bacto-yeast extracts 5 g sodium chloride 10 g
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