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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
广州市左克生物
- CAS号:
2933938-46-4
- 规格:
1mg/5mg
| 规格: | 1mg | 产品价格: | ¥1530.0 |
|---|---|---|---|
| 规格: | 5mg | 产品价格: | ¥3825.0 |
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文献和实验Steady State ATPase Assays Coupled Enzyme System
Tubulin (>5 mg/mL) 100 mM Mg・GTP 4 mM Taxol in DMSO PM = 100 mM PIPES pH 6.8 2 mM EGTA 1 mM Mg2 SO4
Labeling Tubulin and Quantifying Labeling Stoichiometry
to tubulin. We have used it successfully to derivatize tubulin with succinimidyl esters of biotin, digoxigenin, and a wide range of fluorochromes such as tetramethylrhodamine, X-rhodamine, fluorescein, Oregon Green, Cy3, Cy5 and C2CF (bis-caged
by incubation for 45 min. During the incubation, warm the ultracenrtifuge and 50.2Ti rotor to 25oC. Pellet the MTs from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25oC in a 50.2Ti rotor. In the cold
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