- ￥780 - 2040
- Elabscience
- E-AB-F1026M1
- 武汉
- 2026年01月30日
- FCM
- Syrian Hamster
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
CD28抗体
- 抗体英文名：
Elab Fluor® 700 Anti-Mouse CD28 Antibody[37.51]
- 应用范围：
FCM
- 宿主：
Syrian Hamster
- 保质期：
12个月
- 级别：
科研
- 库存：
99
- 供应商：
武汉伊莱瑞特
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态：
液体
- 亚型：
Syrian Hamster IgG
- 规格：
100Tests/50Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥1320.0 |
|---|---|---|---|
| 规格： | 50Tests | 产品价格： | ￥780.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥2040.0 |
Elab Fluor® 700 Anti-Mouse CD28 Antibody[37.51]
| Verified Samples |
Verified Samples in WB: SH-SY5Y
|
| Dilution |
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000
|
| Clonality |
Polyclonal
|
| Isotype |
IgG
|
| Immunogen |
Synthesized peptide derived from the C-terminal region of human Enolase
|
| Abbre |
Enolase
|
| Synonyms |
2 phospho D glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, ENO2, ENOG, Eno 2, Enolase 2, Enolase 2 (gamma, Enolase 2 gamma neuronal, Enolase2, Epididymis secretory protein Li 279, Gamma enolase, Gamma-enolase, HEL S 279, Neural enolase, Neuron , neuronal)
|
| Swissprot |
P09104
|
| Calculated MW |
47 kDa
|
| Observed MW |
47 kDa
|
| Cellular Localization |
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
|
| Concentration |
1 mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
| Buffer |
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
|
| Purification Method |
Affinity purification
|
| Research Areas |
Cancer, Metabolism, Developmental Biology, Neuroscience, Stem Cells, Tags & Cell Markers
|
| Conjugation |
Unconjugated
|
| Background |
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival
|
Elab Fluor® 700 Anti-Mouse CD28 Antibody[37.51]Elabscience®拥有先进的单抗，多抗和流式抗体开发技术平台，提供8500+高品质抗体， 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证，包含 流式细胞术（Flow Cytometry）、 免疫印迹（WB）、 免疫组化（IHC/ICC）、 免疫（共）沉淀（IP/Co-IP）、 免疫荧光（IF） 等实验。抗体产品涉及多种生命科学研究领域，包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类，以保障并加快您的研究进程。
Elab Fluor® 700 Anti-Mouse CD28 Antibody[37.51]This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Elab Fluor® 700 Anti-Mouse CD28 Antibody[37.51]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验分钟收集细胞； 5. 将上一步离心得到的细胞沉淀，重悬至扩增培养基中，并进行计数。 1.3 细胞刺激（次日） 扩增培养基成分：RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28（克隆号：37.51） + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml（本次实验为 2×10^6/ml，该浓度偏大）； 2. 从 4°C 冰箱取出前一天包被的板子，弃掉包被液，无菌PBS洗涤3遍； 3. 每孔加入 500μl 细胞悬液，放置于 37°C 含 5% CO2 细胞
using Dynabeads® CD3⁄CD28 CTS™. Following separation, the CD3 T cells are cultured in the presence of the beads. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads will provide both the primary and co-stimulatory signals
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
with 30 U/ml rIL-2 in a suitable culture plate or tissue culture flask. 2) Add Dynabeads Mouse T-Activator CD3/CD28 at a bead-to-cell ratio of 1:1. 3) Add 30 U/ml rIL-2. 4) Incubate in a humidified CO2 incubator at 37
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