RBFOX3 Polyclonal Antibody

Verified Samples in WB: U87, SH-SY5Y, Human brain, Mouse brain, Mouse cortex, Mouse hippocampal, Rat brain, Rat cortex, Rat hippocampal

WB 1:500-1:2000

Polyclonal

IgG

Recombinant protein corresponding to Mouse NEUN

RBFOX3

FLJ56884, FLJ58356, FOX3NeuN, Fox-1 homolog C, Fox3, HRNBP3, NEUN, RFOX3, RNA binding protein, RNA binding protein fox-1 homolog 3, Rbfox3, fox 1 homolog (C. elegans) 3, fox1 homolog C, hexaribonucleotide binding protein 3, neuronal nuclei

A6NFN3, Q8BIF2

46-48 kDa

46-48 kDa

Cytoplasm, Nucleus.

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH 7.4.

Affinity purification

Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Tags and Cell Markers

Unconjugated

Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.