- ￥1462.50 - 4550
- MCE
- HY-P71496
- 2026年01月22日
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- 规格：
20μg/100μg
| 规格： | 20μg | 产品价格： | ￥1462.5 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥4550.0 |
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文献和实验Expression of a cDNA in E.coli in order to prepare an antiserum
an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase ( LacZ
Analysis of total E.coli protein by SDS PAGE
---------------------------------------------------------------------------- 1 X SDS PAGE loading buffer NuPAGE loading buffer (120 microliters): 30 microliters 4X NuPAGE loading buffer (heat in warm H2O for a few minutes) 60 microliters 0.1 M DTT (16 mg/ml) 30 microliters H2O
Expression of a cDNA in E.coli in order to prepare an antise
and prepare an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase (LacZ ), histidine tags
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