- ￥3900
- biorbyt
- 0.32-20 ng/mL
- 0.117 ng/mL
- orb1146782
- 英国
- 2026年01月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.117 ng/mL
- 规格：
48 T
产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Cytosolic Factor 2(NCF2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neutrophil Cytosolic Factor 2(NCF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Cytosolic Factor 2(NCF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Cytosolic Factor 2(NCF2) in the samples is then determined by comparing the OD of the samples to the standard curve.
产品别名：NOXA2, p67phox, 65kDa, Chronic Granulomatous Disease,Autosomal 2, NADPH Oxidase Activator 2, 67 kDa neutrophil oxidase factor, Neutrophil NADPH oxidase factor 2
应用笔记：standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NCF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NCF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NCF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NCF2 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:P19878
Note：For research use only.
产品别名：NOXA2, p67phox, 65kDa, Chronic Granulomatous Disease,Autosomal 2, NADPH Oxidase Activator 2, 67 kDa neutrophil oxidase factor, Neutrophil NADPH oxidase factor 2
应用笔记：standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NCF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NCF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NCF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NCF2 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:P19878
Note：For research use only.
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Human Neutrophil Cytosolic Factor 2 (NCF2) ELISA Kit试剂盒，orb1146782，biorbyt
￥3900
