- ￥3900
- biorbyt
- 15.63-1000 pg/mL
- 6.2 pg/mL
- orb1146804
- 英国
- 2026年01月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
15.63-1000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
6.2 pg/mL
- 规格：
48 T
产品别名：Probable E3 ubiquitin-protein ligase makorin-3, RING finger protein 63, Zinc finger protein 127, D15S9, RNF63, ZNF127
应用笔记：standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MKRN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MKRN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MKRN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MKRN3 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:Q13064
Note：For research use only.
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文献和实验. For example, novel DNA-binding proteins have been created by using phage display to alter the DNA-binding specificity of a zinc-finger protein (ZFP)2-7 . Although phage display is an effective method, it is labor-intensive and time-consuming
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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