Human Sphingosine 1 Phosphate

产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Lyase 1(SGPL1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Lyase 1(SGPL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Lyase 1(SGPL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Lyase 1(SGPL1) in the samples is then determined by comparing the OD of the samples to the standard curve.

产品别名：SPL, S1PL, Sphingosine-1-phosphate aldolase

应用笔记：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SGPL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SGPL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SGPL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SGPL1 in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：3.5h

UniProt ID:O95470

Note：For research use only.