- ￥3900
- biorbyt
- 1.57-100 ng/mL
- 0.62 ng/mL
- orb781304
- 英国
- 2026年01月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
1.57-100 ng/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Human
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.62 ng/mL
- 规格：
48 T
产品别名：LR8 ELISA Kit
应用笔记：standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TMEM176B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMEM176B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TMEM176B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMEM176B in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:Q3YBM2
Note：For research use only.
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
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BRINK, Maaike EVERTZEN, Gwenda N. PYNAERT,Sander J. H. VAN DEVENTER, et al. A new phosphospecific cell-based ELISA for p42/p44 mitogen-activated protein kinase (MAPK), p38 MAPK, protein kinase B and cAMP-response-element-binding protein. Biochem. J 2000
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