Mouse S100 Calcium Binding Pro

产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A16(S100A16).

产品别名：S100-A16 ELISA Kit, AAG13 ELISA Kit, DT1P1A7 ELISA Kit, S100F ELISA Kit, S100-F ELISA Kit, Aging-associated gene 13 protein ELISA Kit

应用笔记：standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100A16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S100A16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S100A16 in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：3.5h

UniProt ID:Q9D708

Note：For research use only.