Human Mitochondrial Pyruvate C

产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Pyruvate Carrier Protein 1(MPC1).

产品别名：CGI-129 ELISA Kit, BRP44L ELISA Kit, Brain Protein 44 Like Protein ELISA Kit

应用笔记：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MPC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MPC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPC1 in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：3.5h

UniProt ID:Q9Y5U8

Note：For research use only.