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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 应用:
ELISA
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.117 ng/mL
- 规格:
48 T
产品别名:DLG7 ELISA Kit, DLG1 ELISA Kit, HURP ELISA Kit, Discs large homolog 7 ELISA Kit, Disks large-associated protein DLG7 ELISA Kit, Hepatoma up-regulated protein ELISA Kit
应用笔记:standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DLGAP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DLGAP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DLGAP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DLGAP5 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:3.5h
UniProt ID:Q15398
Note:For research use only.
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文献和实验Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
_004781 Homo sapiens vesicle-associated membrane protein 3 (cellubrevin) (VAMP3), mRNA 684 NM_003801 Homo sapiens GPAA1P anchor attachment protein 1 homolog (yeast) (GPAA1), mRNA 776 NM_004643 Homo sapiens poly(A) binding protein, nuclear 1 (PABPN1







