Horse Interleukin 12 p40 (IL-1

产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B(IL12B).

产品别名：p40 ELISA Kit, IL12-B ELISA Kit, CLMF2 ELISA Kit, NKSF2 ELISA Kit, Natural Killer Cell Stimulatory Factor 2 ELISA Kit, Cytotoxic Lymphocyte Maturation Factor 2 ELISA Kit, Interleukin 23, P40 Subunit ELISA Kit

应用笔记：standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：3.5h

Note：For research use only.