- ￥3900
- biorbyt
- 15.63-1000 pg/mL
- 6.6 pg/mL
- orb779671
- 英国
- 2026年01月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
15.63-1000 pg/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Guinea pig
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
6.6 pg/mL
- 规格：
48 T
产品别名：IFNα1 ELISA Kit, IFL ELISA Kit, LeIF D ELISA Kit, IFN ELISA Kit, IFN-Alpha ELISA Kit, IFNα13 ELISA Kit, IFN-A ELISA Kit, IFNαP22 ELISA Kit, IFN-Alpha 1b ELISA Kit, Interferon Alpha 1b ELISA Kit, Interferon, Leukocytic ELISA Kit, IFN, Leukocyte ELISA Kit, Interferon alpha-D ELISA Kit
应用笔记：standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig IFNa in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
Note：For research use only.
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文献和实验CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
部分。它们的错误折叠，如天然结构的丢失，是任何分析的一个重大问题，因为它会引起抗体结合部位、抗体的抗原结合区或抗原的表位的改变，这两者都是待测物结合所必需的。因此，捕获分子无法结合待测物，甚至可能由于暴露天然未暴露的氨基酸序列和结构而发生非特异性结合，导致诊断试验显示错误的结果。由于表面效应引起的空间结构和构象的改变，抗体在包被过程中会发生错误折叠。在ELISA微孔板中，只有3-10%的包被抗体具有良好的定向性和与待测物结合的功能活性。抗原包被也有类似的情况。在大多数情况下，很少量的活性成分就足以完成一次检测
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physiologic and morphologic changes of emphysema in the guinea pig. Am. Rev. Respir. Dis. 142:1422‐1428. Wright, J.L., Postma, D.S., Kerstjens, H.A.M., Timens, W., Whittaker, P
