Guinea pig Interferon Alpha (I

类别: Assays and Kits

描述: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha(IFNa).

别名: IFNα1 ELISA Kit, IFL ELISA Kit, LeIF D ELISA Kit, IFN ELISA Kit, IFN-Alpha ELISA Kit, IFNα13 ELISA Kit, IFN-A ELISA Kit, IFNαP22 ELISA Kit, IFN-Alpha 1b ELISA Kit, Interferon Alpha 1b ELISA Kit, Interferon, Leukocytic ELISA Kit, IFN, Leukocyte ELISA Kit, Interferon alpha-D ELISA Kit

浓度: 1000 pg/mL

应用说明: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig IFNa in the samples is then determined by comparing the OD of the samples to the standard curve

检测时间: 3.5h

研究领域: Neuroscience; Immunology & Inflammation

备注: For research use only.