Pig Nerve Growth Factor (NGF)

产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor(NGF).

产品别名：NGFB ELISA Kit, Beta-NGF ELISA Kit, HSAN5 ELISA Kit, NGF-B ELISA Kit, Beta-Nerve Growth Factor ELISA Kit

应用笔记：standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig NGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig NGF in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：3.5h

UniProt ID:Q29074

Note：For research use only.