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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Competitive
- 应用:
ELISA
- 适应物种:
Human
- 样本:
serum, plasma, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
4.66 pg/mL
- 规格:
48 T
产品别名:ANH ELISA Kit, ANF ELISA Kit, CDD ELISA Kit, Atrial Natriuretic Factor ELISA Kit, Atrial Natriuretic Hormone ELISA Kit, Atriopeptin ELISA Kit, Cardionatrine ELISA Kit, Cardiodilatin ELISA Kit
应用笔记:standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ANP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANP in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:2h
Note:For research use only.
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文献和实验利钠素家族包括:心房钠尿肽(atrial natriuretic peptide ANP)、脑钠素(brain natriuretic peptide,BNP)、C型利钠素(c-type natriuretic pepdde,CNP)。利钠素家族是肾素-血管紧张素-醛固酮系统的天然拮抗剂,也抑制后叶加压素及交感神经的保钠、保水作用。ANP和BNP两者的作用相似,之前对利钠素与蛛网膜下腔出血(SAH)后的低血钠的关系做过较多的研究,但其与SAH的关系的研究则较少。 心脏收到牵引
肌细胞的细胞浆中。ANP已经分离提纯,并且已能人工合成,其氨基酸序列亦已确定。从动物心房肌获得的这类多肽称为心钠素(cardionatrin)或心房肽(atriopeptin)而从人类心房肌所得者称为人心房利钠多肽(human atrial natriuretic polypeptide,hANP)而ANP 则是它们的通称。 动物实验证明,急性的血容量增加可使ANP释放入血,从而引起强大的利钠和利尿作用。血容量增加可能是通过增高右心房压力,牵张心房肌而使ANP释放的。反之,限制钠、水摄入或减少静脉
,也是医学和生理学研究的一个重大进展。ANF后来也被称为心房利钠多肽(atrial natriuretic polypeptide,ANP)因为已经证明它是一种多肽。 ANP主要存在于哺乳动物其中也包括人的心房肌细胞的细胞浆中。ANP已经分离提纯,并且已能人工合成,其氨基酸序列亦已确定。从动物心房肌获得的这类多肽称为心钠素(cardionatrin)或心房肽(atriopeptin)而从人类心房肌所得者称为人心房利钠多肽(human atrial natriuretic







