- ￥3900
- biorbyt
- 0.32-20 ng/mL
- 0.108 ng/mL
- orb779186
- 英国
- 2026年01月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Rat
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.108 ng/mL
- 规格：
48 T
产品别名：CDw338 ELISA Kit, CD338 ELISA Kit, ABC-G2 ELISA Kit, MRX ELISA Kit, BCRP1 ELISA Kit, ABC15 ELISA Kit, ABCP ELISA Kit, BCRP ELISA Kit, BMDP ELISA Kit, MXR ELISA Kit, MXR1 ELISA Kit, Mitoxantrone resistance-associated protein ELISA Kit, Breast Cancer Resistance Protein 1 ELISA Kit, Urate exporter ELISA Kit
应用笔记：standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ABCG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ABCG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ABCG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ABCG2 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:Q80W57
Note：For research use only.
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文献和实验Human ATP-binding cassette (ABC) transporter ABCG2 (BCRP) is critically involved in multidrug resistance of human cancer. This transporter exhibits broad substrate specificity toward structurally diverse compounds, as do other ABC
Clinical studies have strongly suggested that genetic polymorphisms and/or mutations of certain ATP-binding cassette (ABC) transporter genes might be regarded as significant factors affecting patients’ responses to medication and/or the risk
Assessment of Drug Transporter Function Using Fluorescent Cell Imaging
., Ambudkar, S.V., Neubauer, A., and Bates, S.E. 2010. Comparison of ATP‐binding cassette transporter interactions with the tyrosine kinase inhibitors imatinib, nilotinib, and dasatinib. Drug Metab. Dispos. 38:1371‐1380.
