- ￥4290
- biorbyt
- 15.63-1000 ng/mL
- 6.83 ng/mL
- orb777253
- 英国
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
15.63-1000 ng/mL
- 检测方法：
Competitive
- 应用：
ELISA
- 适应物种：
Human
- 样本：
Serum, plasma, other biological fluids
- 灵敏度：
6.83 ng/mL
- 规格：
48 T
产品别名：KLK-L5 ELISA Kit, KLKL5 ELISA Kit, Kallikrein-Related Peptidase 12 ELISA Kit, Kallikrein-like protein 5 ELISA Kit
应用笔记：standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK12 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：2h
UniProt ID:Q9UKR0
Note：For research use only.
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文献和实验低至0.12 ng/mL灵敏度，Elabscience未包被人内皮抑素（ES） elisa试剂盒精准捕捉低丰度样本变化
试剂盒 货号：E-UNEL-H0382 规格：96 T × 5 / 96 T × 15 反应性：人（Human） 检测范围：0.16 - 10 ng/mL 灵敏度：0.12 ng/mL 样本类型：血清、血浆或其他生物体液 检测方法：双抗体夹心法（Sandwich-ELISA），比色法检测 反应时间：24小时 储存与有效期：-20℃保存，有效期12个月 检测原理：双抗体夹心法定量分析 未包被人内皮抑素（ES） elisa试剂盒采用经典的双抗体夹心ELISA原理： 包被
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
准的微量滴定板为8×12的96孔式。为便于作少量标本的检测，有制成8联孔条或12联孔条的，放入座架后，大小与标准ELISA板相同。ELISA板的特点是可以同时进行大量标本的检测，并可在特制的比色计上迅速读出结果。现在已有多种自动化仪器用于微量滴定板型的ELISA检测，包括加样、洗涤、保温、比色等步骤，对操作的标准化极为有利。聚苯乙烯经射线照射后，其吸附性能特别是对免疫球蛋白的吸附性能增加，应用于双抗体夹心法可使固相上抗体量增多，但用于间接法测抗体时空白值较大。良好的ELISA板应该是吸附性能好，空白
