- ￥4290
- biorbyt
- 15.63-1000 ng/mL
- 6.83 ng/mL
- orb777253
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
15.63-1000 ng/mL
- 检测方法：
Competitive
- 应用：
ELISA
- 适应物种：
Human
- 样本：
Serum, plasma, other biological fluids
- 灵敏度：
6.83 ng/mL
- 规格：
48 T
产品别名：KLK-L5 ELISA Kit, KLKL5 ELISA Kit, Kallikrein-Related Peptidase 12 ELISA Kit, Kallikrein-like protein 5 ELISA Kit
应用笔记：standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK12 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：2h
UniProt ID:Q9UKR0
Note：For research use only.
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文献和实验ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
准的微量滴定板为8×12的96孔式。为便于作少量标本的检测，有制成8联孔条或12联孔条的，放入座架后，大小与标准ELISA板相同。ELISA板的特点是可以同时进行大量标本的检测，并可在特制的比色计上迅速读出结果。现在已有多种自动化仪器用于微量滴定板型的ELISA检测，包括加样、洗涤、保温、比色等步骤，对操作的标准化极为有利。聚苯乙烯经射线照射后，其吸附性能特别是对免疫球蛋白的吸附性能增加，应用于双抗体夹心法可使固相上抗体量增多，但用于间接法测抗体时空白值较大。良好的ELISA板应该是吸附性能好，空白
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
