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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
1.57-100 ng/mL
- 检测方法:
Sandwich
- 应用:
ELISA
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
0.48 ng/mL
- 规格:
48 T
产品别名:Apo-M ELISA Kit, G3a ELISA Kit, NG20 ELISA Kit, Protein G3a ELISA Kit
应用笔记:standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOM in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:3.5h
UniProt ID:O95445
Note:For research use only.
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文献和实验80150ml of PBS , 0.01 M, PH 7.4Reference/More informationVoller A , Bartlett A, Bidwell DE, Clark MF, Adams An, 1976. The detection of viruses by enzyme-linked immunosorbent assay (ELISA). J Gen Virol 33, 165-167.Figure 1
pneumoniae , has necessitated a reliance by diagnostic laboratories on serological techniques that are beyond the scope of this text but are summarized in Table 1 . Table 1 An Evaluation of Serological Tests for M. Pneumoniae
Production and Characterization of Recombinant Human and Murine TNF
and the identity of the final products can be checked by SDS-PAGE, ELISA, Western blot, analytical gel-filtration chromatography, mass spectrometry, and lipopolysaccharide assay. The biological activity of both human and murine TNF is assessed by in vitro cytolytic







