- ￥3900
- biorbyt
- 0.32-20 ng/mL
- 0.109 ng/mL
- orb776773
- 英国
- 2026年01月21日
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- 详细信息
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Mouse
- 样本：
urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.109 ng/mL
- 规格：
48 T
产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nephrin(NPHN).
产品别名：NPHS1 ELISA Kit, CNF ELISA Kit, Nephrosis 1 ELISA Kit, Congenital Finnish Type ELISA Kit, Renal glomerulus-specific cell adhesion receptor ELISA Kit
应用笔记：standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NPHN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPHN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NPHN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPHN in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:Q9QZS7
Note：For research use only.
产品别名：NPHS1 ELISA Kit, CNF ELISA Kit, Nephrosis 1 ELISA Kit, Congenital Finnish Type ELISA Kit, Renal glomerulus-specific cell adhesion receptor ELISA Kit
应用笔记：standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NPHN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPHN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NPHN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPHN in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:Q9QZS7
Note：For research use only.
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Mouse Nephrin (NPHN) ELISA Kit试剂盒，orb776773，biorbyt
￥3900
