Mouse Nicotinamide Adenine Din
ucleotide Phosphate Oxidase 4 (NOX4) ELISA Kit试剂盒，orb776646，biorbyt- ￥3900
- biorbyt
- 0.16-10 ng/mL
- 0.057 ng/mL
- orb776646
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Mouse
- 样本：
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.057 ng/mL
- 规格：
48 T
产品别名：KOX ELISA Kit, KOX-1 ELISA Kit, RENOX ELISA Kit, NADPH Oxidase 4 ELISA Kit
应用笔记：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NOX4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NOX4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NOX4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NOX4 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:Q9JHI8
Note：For research use only.
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文献和实验Monocytes, macrophages, neutrophils, and eosinophils are able to generate and release reactive oxygen species. The reactive oxygen species are generated by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase which is activated
Eosinophils: Collection, Separation, and Activation
phagocytosis, production of toxic oxygen metabolites, initiated by a membrane bound nicotinamide adenine dinucleotide phosphate (NADPH)oxidase, and degranulation of cytotoxic proteins (for reviews see 1 ,2 ). Apart from being cytotoxic, these cells
R) in cardiovascular tissue, and subsequent activation of the nicotinamide adenine dinucleotide phosphate (NADPH) enzymatic complex, which finally leads to increased ROS production. This resulting state of enhanced oxidative stress contributes largely to generalized
