Mouse Glucokinase (GCK) ELISA

产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucokinase(GCK).

产品别名：GK ELISA Kit, GLK ELISA Kit, HHF3 ELISA Kit, HK4 ELISA Kit, HKIV ELISA Kit, HXKP ELISA Kit, MODY2 ELISA Kit, Hexokinase-D ELISA Kit, Hexokinase 4 ELISA Kit, Maturity Onset Diabetes Of The Young 2 ELISA Kit

应用笔记：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GCK in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：3.5h

UniProt ID:P52792

Note：For research use only.