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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 应用:
ELISA
- 适应物种:
Human
- 样本:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.053 ng/mL
- 规格:
48 T
产品别名:DDPAC ELISA Kit, FTDP-17 ELISA Kit, MAPTL ELISA Kit, MSTD ELISA Kit, MTBT1 ELISA Kit, MTBT2 ELISA Kit, PPND ELISA Kit, Neurofibrillary tangle protein ELISA Kit, Tau Protein ELISA Kit, Paired helical filament-tau ELISA Kit, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1 ELISA Kit
应用笔记:standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAPt. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAPt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAPt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAPt in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:3.5h
UniProt ID:P10636
Note:For research use only.
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文献和实验Screening for Inhibitors of Microtubule-Associated Motor Proteins
The mitotic spindle is an important target for cancer chemotherapy. The main protein target for drugs in clinical use is tubulin, the building block of microtubules. In recent years, other proteins of the mitotic spindle have been identified
of microtubule-associated proteins. Tau is strongly expressed in neurons, localized in the axon and is essential for neuronal architecture, plasticity and network. In animal models, overexpression of mutated Tau proteins is often used to induce a Tau pathology
Tau Phosphorylation Both In Vitro and in Cells
Tau was originally isolated from brain microtubules and shown to be a microtubule-associated protein (MAP) that promoted tubulin polymerization (1 ). It is largely confined to axons, where it is the major MAP. It promotes microtubule nucleation
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