Rat Cross Linked C-Telopeptide

产品描述：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cross Linked C-Telopeptide Of Type II Collagen(CTXII) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cross Linked C-Telopeptide Of Type II Collagen(CTXII).

产品别名：CTX-II ELISA Kit, CTX2 ELISA Kit

应用笔记：standard: 8000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat CTXII protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTXII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CTXII in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：2h

Note：For research use only.