Human Thyrotropin Releasing Ho

产品描述：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(TRH).

产品别名：TRF ELISA Kit, Thyrotropin-Releasing Factor ELISA Kit, Thyroliberin ELISA Kit, Protirelin ELISA Kit, Pro-tirelin ELISA Kit, Prothyroliberin ELISA Kit, TSH-releasing factor ELISA Kit

应用笔记：standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRH in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：2h

UniProt ID:P20396

Note：For research use only.