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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
4.69-300 ng/mL
- 检测方法:
Competitive
- 应用:
ELISA
- 适应物种:
All
- 样本:
serum, plasma and other biological fluids
- 灵敏度:
1.48 ng/mL
- 规格:
48 T
产品描述:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroxine(T4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroxine(T4).
产品别名:3,5,3',5'-Tetraiodothyronine ELISA Kit, L-Thyroxine ELISA Kit, Levothyroxine ELISA Kit
应用笔记:standard: 300 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:2h
Note:For research use only.
产品别名:3,5,3',5'-Tetraiodothyronine ELISA Kit, L-Thyroxine ELISA Kit, Levothyroxine ELISA Kit
应用笔记:standard: 300 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:2h
Note:For research use only.
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General Thyroxine (T4) ELISA Kit试剂盒,orb775069,biorbyt
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