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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
4 μmol/L - 400 μmol/L
- 检测方法:
Detection
- 应用:
FA
- 适应物种:
All
- 样本:
Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
- 规格:
100 assay
产品别名:EC 4.1.3.1; Isocitrate lyase; ICL; Isocitrase; Isocitratase; Isocitrate glyoxylate-lyase; Isocitritase
保存说明:Shipped and store at 4°C for 6 months.
Note:For research use only.
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文献和实验Using N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro
activity. Colorimetric assays are often the preferred methods of enzyme analysis since they may be readily adapted to simple microplate formats that require relatively inexpensive and widely available instrumentation. The use of N ,N ,N �,N �-tetramethyl-p
, these assays can readily be performed in a microplate with relatively few cells. Since a cytotoxic factor will reduce the rate of tetrazolium salt cleavage by a population of cells, these metabolic activity assays are frequently used to measure factor-induced
as substrate. Note that most of the DUB activity of 26S in (A) comes from proteasome‐associated UCH37 since RPN11 does not cleave Ub‐AMC. RFU indicates relative fluorescence unit. Detailed descriptions for the assay reagents and the DUB assay
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