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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Acid ceramidase (Asah1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Acid ceramidase (Asah1), partial品名简称:
Recombinant Mouse Asah1 protein, partial货号:
CSB-CF895296MO规格:
20μg价格:
5268LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Asah1Uniprot No.:
Q9WV54别名:
Acylsphingosine deacylase N-acylsphingosine amidohydrolase种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
in vitro E.coli expression system分子量:
33.8 kDa表达区域:
19-141aa氨基酸序列:
QAQDVPPWTEDCRKSTYPPSGPTYRGPVPWHTINLDLPPYKRWHELLAQKAPALRILVNSITSLVNTFVPSGKLMKMVDQKLPGMIGSLPDPFGEEMRGIADVTGIPLGEIISFNIFYELFTM蛋白标签:
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.功能2:
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.文献:
"Molecular cloning and characterization of a human cDNA and gene encoding a novel acid ceramidase-like protein."Hong S.-B., Li C.-M., Rhee H.-J., Park J.-H., He X., Levy B., Yoo O.J., Schuchman E.H.Genomics 62:232-241(1999)研究领域:
Signal Transduction请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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,往往无法真实模拟人类Siglec-8或Siglec-9在人体内的实际生物学功能。研究者须进一步评估小鼠模型所得结论向临床转化的可靠性。 从科学问题到工具选择 当您确定了研究的 Siglec 成员和应用场景后,接下来的挑战是选择能够支撑高质量数据的实验工具。研究工具的选择应直接回馈您的实验问题: 如果您的问题是“受体能否识别特定的聚糖配体”:您需要具有生物活性的重组蛋白(如 Fc 融合蛋白或构象稳定的 His 标签蛋白)。这种工具是 SPR(表面
不只是 PD-1:CD96 正成为免疫治疗的下一个“必争之地”
状态密切相关,也是理解其生物学作用的重要基础。 3. CD96 信号通路与作用机制:配体结合如何调控免疫反应 3.1 CD96 与配体 CD155 的结合机制 CD96 的核心功能主要通过与配体 CD155(PVR)的结合介导。研究已明确证实,CD96 可直接与 CD155 相互作用,这一结合是其启动下游信号转导、调节免疫细胞功能的关键基础 [7]。从结构角度看,CD96 是典型的 I 型跨膜糖蛋白,其胞外区含有多个 Ig 样结构域,其中 N 端 IgV 结构域被认为是与 CD155
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