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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Biotin--protein ligase (HLCS)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Biotin--protein ligase (HLCS)品名简称:
Recombinant Human HLCS protein货号:
CSB-EP010518HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
HLCSUniprot No.:
P50747别名:
Biotin apo-protein ligase种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
85.8 kDa表达区域:
1-726aa氨基酸序列:
MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Biotin--protein ligase catalyzing the biotinylation of the 4 biotin-dependent carboxylases acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, and methylcrotonyl-CoA carboxylase功能2:
/文献:
/研究领域:
Signal Transduction请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验QIAgene Expression Kit——再也不为重组蛋白的表达效率发愁 据Protein Database的最新数据表明,目前已知的人类蛋白有35,000种(包括splice variants),大约33%的蛋白正处于研究中,但是只有8%的蛋白能够成功表达。统计数据同时显示,目前克隆,表达,纯化的成功率只维持在54%,74%,59%。所以说,在蛋白表达的漫漫征途中,拿不到想要的蛋白是一件非常普遍的事情。举一个例子来说对于目标蛋白不表达或表达量低这个常见问题,需要排除或检查的因素就太多
binding protein 1 (ZBP1) 被认为是病毒感染诱导炎症和程序性细胞死亡 (尤其是坏死性凋亡) 的关键上游调控因子。然而,针对 ZBP1 的特异性抑制剂或降解策略长期缺乏,严重限制了其在抗炎和抗病毒治疗中的转化应用。 传统药物 & PROTAC ZBP1 结构特殊、缺乏成熟的小分子配体,长期被视为“难成药靶点”。蛋白降解靶向嵌合体 (PROTAC) 技术的兴起带来了转机。与传统药物“占据并抑制”蛋白功能的思路不同,PROTAC 像一座 “分子桥梁
human full length protein coding ORFs ; ; ORF2: ; ;eGFP eCFP eYFP Luciferase Biotin Ligase ; ;UBC9 HA-Sumo His-AviTag-ubiquitin Neomycin Human ORFs zhaolifei认为: 不知道楼主注意到没有:质粒的不相容性,具有相同复制起始
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