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- 保存条件:
见瓶身
- 保质期:
见瓶身
- 英文名:
O-ALPHA-D-甘露糖基-(1-3)-O-[O-ALPHA-D-甘露糖基-(1-3)-O-[ALPHA-D-甘露糖基-(1-6)]-ALPHA-D-甘露糖基-(1-6)]-O-BETA-D-甘露糖基-(1-4)-O-2-(乙酰氨基)-2-脱氧-BETA-D-吡喃葡萄糖基-(1-4)-2-(乙酰氨基)-2-脱氧-D-葡萄糖
- 库存:
999
- 供应商:
麦格生物
- CAS号:
66091-47-2
- 规格:
10μg
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文献和实验Replication timing by density transfer
Calbiochem's Pronase (catalog #53702), which seems pretty clean--i.e., plates spread after Pronase treatment aren't contaminated--but I heard from someone who'd used Sigma's Pronase and had massive growth of Streptomyces. 20x SCP = 0.6 M Na2 HPO
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
References 1. R. M. O’Connell, K. D. Taganov, M. P. Boldin, G. Cheng, D. Baltimore, MicroRNA-155 is induced during the macrophage inflammatory response. Proc. Natl. Acad. Sci. U.S.A. 104, 1604–1609 (2007).[Abstract/Free Full Text] 2. D
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