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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
62.5-4000pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
37.5 pg/mL
- 规格:
48 T
产品别名:ALXR, FMLP-R-II, FMLPX, FPR2A, FPRH1, FPRH2, FPRL1, HM63, LXA4R, N-formyl peptide receptor 2
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FPR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FPR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FPR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FPR2. You can calculate the concentration of Human FPR2 in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P25090
靶点:FPR2
Note:For research use only.
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
Detection of High-Affinity 4-Integrin Upon Leukocyte Stimulation by Chemoattractants or Chemokines
the leucine, aspartic acid, valine (LDV) consensus binding sequence for α4-integrin, is described (8 ). The cell line U937, transfected with the human formyl peptide receptor (FPR), and stimulated with fMLP or SDF-1α is used as a model. These methods allow







