- ￥3510
- biorbyt
- 0.16-10ng/mL
- 0.1 ng/mL
- orb1948820
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.16-10ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.1 ng/mL
- 规格：
48 T
产品别名：SLEB1
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IRF5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IRF5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IRF5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IRF5. You can calculate the concentration of Mouse IRF5 in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P56477
靶点：IRF5
Note：For research use only.
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文献和实验【信息】The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling
with the exception of TLR3. MyD88 couples to pathways that lead to the activation of transcription factors such as NF-B (nuclear factor-B), IRF1 (IFN-regulatory factor 1), IRF5 and IRF7. It is also involved in TLR-independent signals activated by IL-1, IL-18 and IFN
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
CalbiochemReferenceWeskamp, G., and Otten, U. (1987). An enzyme-linked immunoassay for nerve growth factor (NGF): A tool for studying regulatory mechanisms involved in ngf production in brain and in peripheral tissues. Journal of Neurochemistry, 48 (6),1779-1786.
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