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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.32-20ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
0.19 ng/mL
- 规格:
48 T
产品别名:ODC1
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ODC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ODC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ODC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ODC. You can calculate the concentration of Human ODC in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P11926
靶点:ODC
Note:For research use only.
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文献和实验Posttranscriptional Regulation of Ornithine Decarboxylase
Activity of the polyamine biosynthetic enzyme ornithine decarboxylase (ODC) and intracellular levels of ODC protein are controlled very tightly. Numerous studies have described ODC regulation at the levels of transcription, translation
Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine
L -Ornithine decarboxylase (EC 4.1.1.17) (ODC) catalyzes the conversion of L -ornithine to putrescine and CO2 . ODC is dependent on pyridoxal 5′-phosphate (PLP) and thiol-reducing agents for activity (1 ). At least two key active site
Determination of Ornithine Decarboxylase Activity Using [3H]Omithine
Ornithine decarboxylase (EC 4.1.1.17) (ODC) catalyzes the decarboxylation of ornithine to form putrescine. This reaction is the rate-limiting step in the biosynthesis of the naturally occurring polyamines, which regulate proliferation
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