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Rat Heat Shock Protein 27 (HSP

-27/HSPB1) ELISA Kit试剂盒,orb1948583,biorbyt
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  • ¥3770
  • biorbyt已认证
  • 0.79-50ng/mL
  • 0.47 ng/mL
  • orb1948583
  • 英国
  • 2026年01月21日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 供应商

      biorbyt

    • 检测范围

      0.79-50ng/mL

    • 检测方法

      Sandwich

    • 适应物种

      Rat

    • 样本

      serum, plasma, Tissue homogenate and Other biological samples

    • 灵敏度

      0.47 ng/mL

    • 规格

      48 T

    产品描述:Rat Heat Shock Protein 27 (HSP-27/HSPB1) ELISA Kit

    产品别名:HSPB1, CMT2F, HMN2B, HS.7667, HSP27, HSP28, Hsp25, SRP27

    应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-27/HSPB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-27/HSPB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-27/HSPB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSP-27/HSPB1. You can calculate the concentration of Rat HSP-27/HSPB1 in the samples by comparing the OD of the samples to the standard curve.

    实验时长:3.5H

    靶点:HSP-27/HSPB1

    Note:For research use only.

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    图标文献和实验
    相关实验
    • Analysis of Heat-Shock Protein Localisation Using Flow Cytometry

      The use of flow cytometry in heat-shock protein (HSP) research is increasing rapidly due to the high sensitivity and versatility of the technique. The method allows the simultaneous analysis of multiple proteins within numerous cell types

    • StructureFunctions of HspB1 (Hsp27)

      Human HspB1 (also denoted Hsp27) is a well-known member, together with alphaB-crystallin, of the small heat-shock (or stress) proteins (sHsps) (20–40 kDa). In this chapter, I describe procedures for testing the oligomeric and phosphorylation

    • Quantification of HSP27 and HSP70 Molecular Chaperone Activities

      Stress-inducible heat-shock proteins (HSPs, like HSP70 and HSP27) are molecular chaperones that �protect cells from stress damage by keeping cellular proteins in a folding competent state and preventing them from irreversible aggregation. HSP27

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