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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
15.63-1000pg/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
9.38 pg/mL
- 规格:
48 T
产品别名:G34, Gastrin component II, Big gastrin
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GT-34. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GT-34 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GT-34, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GT-34. You can calculate the concentration of Mouse GT-34 in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
靶点:GT-34
Note:For research use only.
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文献和实验In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model
might become a limiting factor when treating children with inherited disorders, due to the low number of bone marrow (BM) CD34+ HSCs that can actually be isolated. These problems could be overcome by using efficient in vivo HSC-specific lentiviral vectors (LVs
CML Mouse Model in Translational Research
(1) with the Philadelphia chromosome resulting from of a reciprocal translocation between the chromosomes 9 and 22 t(9;22)-(q34;q11). This translocation produces a fusion gene known as BCR-ABL which acquires uncontrolled tyrosine kinase activity, constantly turning
°C for several hrs or at 4°C overnight. Completely aspirate collagen from the dish before adding cells. Note: Do not allow Vitrogen stock to warm, or it will begin to gel. Culture mouse keratinocytes at 33-34°C, 8% CO2







