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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.32-20ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
0.19 ng/mL
- 规格:
48 T
产品别名:GLP1R, entrez:274, glucagon-like peptide 1 receptor, GLP-1, GLP-1-R, GLP-1R, glucagon like peptide 1 receptor
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GLP1R. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLP1R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLP1R, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GLP1R. You can calculate the concentration of Human GLP1R in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:P43220
靶点:GLP1R
Note:For research use only.
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文献和实验三句话读懂一篇 CNS:橙子可甜可酸的原因;吃红肉促进结直肠癌发生的分子机制...
论文 Molecular insights into ago-allosteric modulation of the human glucagon-like peptide-1 receptor,首次报道了 Compound 2 结合 GLP-1R 的三个高分辨率冷冻电镜结构,突破技术瓶颈, 揭示了该受体变构激动调节的分子机制,为拓展受体药理学内涵和相关药物发现提供了重要的结构基础。 图 10. 来源 Nature Communications 本周推荐:三句话读懂一篇 CNS:男性的大脑和睾丸相似;父亲熬夜,影响
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic







