- ￥3770
- biorbyt
- 0.63-40ng/mL
- 0.38 ng/mL
- orb1947290
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.63-40ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.38 ng/mL
- 规格：
48 T
产品别名：TFRC, CD71, T9, TFR, TFR1, TR, TRFR, p9, IMD46, transferrin receptor
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TFR1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TFR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TFR1. You can calculate the concentration of Mouse TFR1 in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:Q62351
靶点：TFR1
Note：For research use only.
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文献和实验ELISA-based assay for scatchard analysis of ligand-receptor interactions
transferrin to its receptors on mouse fibroblasts and yieldedK d values similar to those obtained with other published methods. The assay is sensitive, rapid, and also convenient, because aliquots of semi-intact cells can be stored frozen. The perforated
secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
