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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Ribonuclease H1 (RNASEH1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Ribonuclease H1 (RNASEH1)品名简称:
Recombinant Human RNASEH1 protein货号:
CSB-EP019801HUc7规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
RNASEH1Uniprot No.:
O60930别名:
RNase H1;Ribonuclease H type II种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
39 kDa表达区域:
1-286aa氨基酸序列:
MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.功能2:
/文献:
/研究领域:
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文献和实验The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides
Ribonuclease H (RNase H), first observed in extracts of calf thymus, is an apparently ubiquitous enzyme activity (1 ), which catalyzes the hydrolysis of RNA in RNA-DNA heteroduplexes (2 ). The two main classes (Type I and II) of eukaryotic
% 以上的活性。由于 Prime RNase Inhibitor 是一种蛋白质,所以应该保存于 -20 度,在 -70 度条件下会导致蛋白变性。FAQ :已经加入Prime RNase Inhibitor 后的 RNA 样品应该如何保存?A :应该在 -20 度保存。Prime RNase Inhibitor 成分是什么?是否 HPRI/ 或者 RNasin Ribonuclease Inhibitor 的另一版本?A :是一种非人源蛋白。由于商业秘密不能透露。但是是完全不同类型的另一种蛋白。并非重组蛋白
Measurement of GFP Expression and DNA Content in Permeabilized Cells
Reagents Cells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as a control. 1 X PBS 2% Buffered formaldehyde solution (see recipe) 70% Ethanol Propidium
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