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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli ATP-dependent DNA helicase recQ (recQ), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli ATP-dependent DNA helicase recQ (recQ), partial品名简称:
Recombinant E.coli recQ protein, partial货号:
CSB-EP321342ENV1规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
recQUniprot No.:
P15043别名:
recQ; b3822; JW5855; ATP-dependent DNA helicase RecQ; EC 3.6.4.12种属:
Escherichia coli (strain K12)蛋白长度:
Partial来源:
E.coli分子量:
71.9 kDa表达区域:
2-606aa氨基酸序列:
AQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRPEYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGD蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS syst. It is a DNA helicase.功能2:
Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase.文献:
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)研究领域:
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文献和实验the protein components of cell organelles by the hydrolysis of tissue slices (6 ), and as an alternative to proteinase K to remove protein during plasmid DNA (7 ), chromosomal DNA (8 ), and RNA isolation (9 –11 ). Another use of pronase is the production
筛选,总共破坏了30种癌症的300多个癌症模型的2万个基因,才揭示出哪些基因对生存至关重要,重点研究了常见的肺癌、结肠癌和乳腺癌,以及急需新疗法的胰腺癌和卵巢癌等。数千个关键癌症基因录入一个优先排列系统,输出具备最大药物开发前景的大约600个基因。在多种不同癌症类型中,一个最高评分靶标是Werner综合征RecQ螺旋酶(WRN)。研究小组发现,DNA修复路径有缺陷的癌细胞(又称microsatellite unstable cancers)需要WRN才能存活。其中包括15%的结肠癌和28%的胃癌
Papillomavirus DNA Replication
of replication ( 2 , 3 ). The minimal origin of replication consists of an E1 binding site, an E2 binding site, and an AT rich region that probably facilitates origin unwinding ( see Fig. 1 ). The E1 protein is an ATP-dependent helicase
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