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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Apis mellifera Defensin-1
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Apis mellifera Defensin-1品名简称:
Recombinant Apis mellifera Defensin-1 protein货号:
CSB-EP324652DNK规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P17722别名:
(Royalisin)种属:
Apis mellifera (Honeybee)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
11.6 kDa表达区域:
44-94aa氨基酸序列:
VTCDLLSFKGQVNDSACAANCLSLGKAGGHCEKGVCICRKTSFKDLWDKRF蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Found in royal jelly and in hemolymph, potent antibacterial protein against Gram-positive bacteria at low concentration.功能2:
/文献:
"Two structurally different defensin genes, one of them encoding a novel defensin isoform, are expressed in honeybee Apis mellifera." Klaudiny J., Albert S., Bachanova K., Kopernicky J., Simuth J. Insect Biochem. Mol. Biol. 35:11-22(2005)研究领域:
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相关实验
合成和遗传重组表达的蛋白,他们对其中不同类别的代表性序列进行了结构、功能和进化研究,确定了4个抗微生物肽基因的抗微生物活性,发现γ-core区域是防御肽Navidefensin2-2的抗菌活性表面。他们的结果还表明,基因重复和功能区域的正选择可能驱动了金小蜂防御肽基因家族的适应性进化。 此外,运用比较基因组学的方法,研究人员还发现,与同为膜翅目的意大利蜜蜂(Apis mellifera)相比,金小蜂直系同源的抗微生物肽基因发生了明显的变化。主要表现在基因数量扩张,蛋白质末端延长,功能域的串联重复和融合
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文献支持
//重组蛋白(Royalisin)Apis mellifera Defensin-1蛋白
¥2328






