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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4
- 保质期:
详询
- 英文名:
Protein Carbonyl TCA Solution
- 库存:
10
- 供应商:
北京孚博
- CAS号:
10005847
- 规格:
1 ea
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文献和实验Protein Carbonyl Measurement by ELISA
Protein carbonyls are formed by a variety of oxidative mechanisms and are sensitive indices of oxidative injury (1). The conventional assay for protein carbonyls is a colorimetric procedure that measures binding of dinitrophenylhydrazine (DNP
volume of 20% TCA (trichloroacetic acid)to protein sample.2.Incubate 30 min on ice.3.Spin in microfuge at 4 deg.For 15 min.4.Carefully remove all supernatant.5.Add ~300μl cold acetone and spin 5 min at 4 degrees.6.Remove supernatant and dry pellet.7.Resuspend
Protein Carbonyl LevelsAn Assessment of Protein Oxidation
Oxidative stress may be a hallmark of several neurodegenerative disorders, including Alzheimer’s disease (AD), Huntington’s disease (HD), and Parkinson’s disease (PD), as well as Creutzfeldt-Jakob disease (CJD), frontotemporal dementia
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