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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见COA
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- 规格:
100ug; 500ug; 1mg; Bulk

| 产品信息
品名:NebuSelect™ Recombinant Human LYSMD3 Protein, 61-120aa, His-tag, SUMO-tag
中文名称:重组人LYSMD3蛋白
别名:LYSMD3, LysM and putative peptidoglycan-binding domain-containing protein 3
货号:NBL-296715
品牌:Nebulabio
规格:100UG;1MG;Bulk
| 产品描述
NebuSelect™ Recombinant Human LYSMD3 Protein, His-tag, SUMO-tag (Cat# NBL-296715) is expressed E. coli with His, SUMO Tag. This recombinant protein is of high purity, verified to be greater than 90% by SDS-PAGE. Endotoxin levels are less than 1 EU/µg, as determined by the LAL (Limulus Amebocyte Lysate) assay.
| 产品属性
Species:Human
Expression System:E. coli
Molecular Weight:19 kDa
Target Name:LYSMD3
Purity:>90% by SDS-PAGE.
Formulation:See COA
Endotoxin:<1 EU/ug(LAL Method)
Storage:4°C for short term. -20°C for long term.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验Strategies to Optimize Protein Expression in E. coli
. 2009a. Recombinant expression and affinity purification of a novel epididymal human sperm‐binding protein, BSPH1. Mol. Hum. Reprod. 15:105‐114. Lefebvre, J., Boileau, G
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
on engineered aminopeptidases. Using human tumor necrosis factor α as a model protein, we describe here the steps involved in the removal of a His-tag using TAGZyme. The tag used (UZ-HT15) has been optimized for expression in Escherichia coli and for TAGZyme
的吸附在固相介质上,例如离子交换介质、金属螯合介质等,可以避免伸展的多肽分子之间形成聚集体。目标蛋白上的His-tag [8] 和Cellulose-binding domain[9]在伸展的状态下仍旧保持结合的能力。 最近层析复性方法成为研究的热点,不仅可以降低聚集体的形成,还可以适用于大规模生产,同时对目标蛋白质有纯化作用。根据复性方式的不同,可以把层析复性方法分为三种[10]: 1) 基于缓冲液置换机理的凝胶过滤层析 2) 蛋白质可逆的直接吸附在介质上,随后除去变性剂诱导蛋白
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