- 询价
- Nebulabio
- 中国
- NBL-296668
- 2026年01月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
见COA
- 库存：
大量
- 供应商：
北京辰辉创聚生物技术有限公司
- 规格：
100ug; 500ug; 1mg; Bulk
| 产品信息
品名：NebuSelect™ Recombinant Human CISD1 Protein, 16-108aa, His-tag, SUMO-tag
中文名称：重组人CISD1蛋白
别名：CISD1, CDGSH iron-sulfur domain-containing protein 1, MitoNEET, C10orf70, ZCD1
货号：NBL-296668
品牌：Nebulabio
规格：100UG；1MG；Bulk
| 产品描述
NebuSelect™ Recombinant Human CISD1 Protein, His-tag, SUMO-tag (Cat# NBL-296668) is expressed E. coli with His, SUMO Tag. This recombinant protein is of high purity, verified to be greater than 90% by SDS-PAGE. Endotoxin levels are less than 1 EU/µg, as determined by the LAL (Limulus Amebocyte Lysate) assay.
| 产品属性
Species:Human
Expression System:E. coli
Molecular Weight:23 kDa
Target Name:CISD1
Purity:>90% by SDS-PAGE.
Formulation:See COA
Endotoxin:＜1 EU/ug(LAL Method)
Storage:4°C for short term. -20°C for long term.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
on engineered aminopeptidases. Using human tumor necrosis factor α as a model protein, we describe here the steps involved in the removal of a His-tag using TAGZyme. The tag used (UZ-HT15) has been optimized for expression in Escherichia coli and for TAGZyme
.16 112.05 Dynabeads M-280 Streptavidin Supplied at 6.7x108 beads per ml (10mg/ml) in PBS, pH7.4 with 0.1% BSA and 0.02% sodium azide. Isolation of any biotinylated target. Applications include DNA, RNA and protein isolation
缓冲液为破菌缓冲液。对于不可溶表达的重组蛋白，最简单的就是选用PBS为破菌缓冲液。 在选用破菌缓冲液时，有个小小的trick，加EDTA。有个别重组蛋白很脆弱，在超声破菌时就有大量的降解。注意，不是表达降解，而是超声过程中降解。特别是用pET32表达的his-tag融合蛋白容易降解，我运气好，遇到过2次这种超声降解。第一次碰到时，害得我好苦。反复找原因，是诱导表达温度？是超声温度过高？超声功率？外源蛋白酶污染？……最后用EDTA解决了。在破菌缓冲液中加0.5mM的EDTA就不会降解了。推测原因
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