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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见COA
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- 规格:
100ug; 500ug; 1mg; Bulk

| 产品信息
品名:NebuSelect™ Recombinant Human PRAP1 Protein, 21-151aa, His-tag, KSI-tag
中文名称:重组人PRAP1蛋白
别名:Proline-rich acidic protein 1, Epididymis tissue protein Li 178, Uterine-specific proline-rich acidic protein, PRAP1, UPA, UNQ608/PRO1195
货号:NBL-293420
品牌:Nebulabio
规格:100UG;1MG;Bulk
| 产品描述
NebuSelect™ Recombinant Human PRAP1 Protein, His-tag, KSI-tag (Cat# NBL-293420) is expressed E. coli with His, KSI Tag. This recombinant protein is of high purity, verified to be greater than 90% by SDS-PAGE. Endotoxin levels are less than 1 EU/µg, as determined by the LAL (Limulus Amebocyte Lysate) assay.
| 产品属性
Species:Human
Expression System:E. coli
Molecular Weight:31 kDa
Target Name:PRAP1
Purity:>90% by SDS-PAGE.
Formulation:See COA
Endotoxin:<1 EU/ug(LAL Method)
Storage:4°C for short term. -20°C for long term.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验Strategies to Optimize Protein Expression in E. coli
. 2009a. Recombinant expression and affinity purification of a novel epididymal human sperm‐binding protein, BSPH1. Mol. Hum. Reprod. 15:105‐114. Lefebvre, J., Boileau, G
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
on engineered aminopeptidases. Using human tumor necrosis factor α as a model protein, we describe here the steps involved in the removal of a His-tag using TAGZyme. The tag used (UZ-HT15) has been optimized for expression in Escherichia coli and for TAGZyme
。 (一)、可溶表达的蛋白A 1、computer paper work: 大肠杆菌BL21,pET32a载体融合表达,N端融合Trx(硫氧还蛋白109aa)和his6-tag(共49aa),DDDDK肠激酶位点后为需要的目标蛋白A,融合蛋白的分子量为21.2kd,Trx(DDDDK前)的分子量为17.1kd,蛋白A的分子量为4.1kd,蛋白A稳定性好,水溶性好,融合蛋白的理论等电点为5.9,Trx(DDDDK前158aa)的理论等电点为5.4,蛋白A的理论等电点为9.7。 2、纯化要求 符合
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