- ￥1763
- SIGMA
- 美国
- 51602-25MG
- 2026年01月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
见瓶身
- 英文名：
Sudan Red B
- 库存：
999
- 供应商：
麦格生物
- CAS号：
3176-79-2
- 规格：
25mg
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文献和实验temperature 45 minutes 8. Wash in water 10 minutes 9. Differentiate in Acid alcohol 10. Wash in water 10 minutes 1. Stain in Picro-sirius red 1 hour 12. Wash in water 13. Dehydrate, clear . Mount sections in DPX N.B. Wash quickly
were performed in electrocompetent DH10B or HS996 cells harbouring the pSC101BADγβαA[hygro] or the pSC101BADγβαA-I-SceI[amp] plasmids and using standard Red/ET protocols (8). Electrocompetent cells were prepared as described in the general manuals
Recombineering/Lambda red-mediated gene replacement
Overview Single-gene knockouts using λ red system, adapted from Datsenko and Wanner paper. The goal of this protocol was to create an endA (endonuclease I) knockout, but obviously it can be adapted to any gene. The knocked-out gene
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